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Multisite Electrophysiology Recordings in Mice to Study Cross-Regional Communication During Anxiety.


ABSTRACT: Recording neural activity in awake, freely moving mice is a powerful and flexible technique for dissecting the neural circuit mechanisms underlying pathological behavior. This unit describes protocols for designing a drive and recording single neurons and local field potentials during anxiety-related paradigms. We also include protocols for integrating pharmacologic and optogenetic means for circuit manipulations, which, when combined with electrophysiological recordings, demonstrate input-specific and cell-specific contributions to circuit-wide activity. We discuss the planning, execution, and troubleshooting of physiology experiments during anxiety-like behavior. © 2017 by John Wiley & Sons, Inc.

SUBMITTER: Harris AZ 

PROVIDER: S-EPMC5783183 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Multisite Electrophysiology Recordings in Mice to Study Cross-Regional Communication During Anxiety.

Harris Alexander Z AZ   Golder Danielle D   Likhtik Ekaterina E  

Current protocols in neuroscience 20170705


Recording neural activity in awake, freely moving mice is a powerful and flexible technique for dissecting the neural circuit mechanisms underlying pathological behavior. This unit describes protocols for designing a drive and recording single neurons and local field potentials during anxiety-related paradigms. We also include protocols for integrating pharmacologic and optogenetic means for circuit manipulations, which, when combined with electrophysiological recordings, demonstrate input-speci  ...[more]

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