Dataset Information

Enzyme-Induced Matrix Softening Regulates Hepatocarcinoma Cancer Cell Phenotypes.

ABSTRACT: The progression of cancer is often accompanied by changes in the mechanical properties of an extracellular matrix. However, limited efforts have been made to reproduce these biological events in vitro. To this end, this study demonstrates that matrix remodeling caused by matrix metalloproteinase (MMP)-1 regulates phenotypic activities and modulates radiosensitivity of cancer cells exclusively in a 3D matrix. In this study, hepatocarcinoma cells are cultured in a collagen-based gel tailored to present an elastic modulus of ?4.0 kPa. The subsequent exposure of the gel to MMP-1 decreases the elastic modulus from 4.0 to 0.5 kPa. In response to MMP-1, liver cancer cells undergo active proliferation, downregulation of E-cadherin, and the loss of detoxification capacity. The resulting spheroids are more sensitive to radiation than the spheroids cultured in the stiffer gel not exposed to MMP-1. Overall, this study serves to better understand and control the effects of MMP-induced matrix remodeling.

SUBMITTER: Liang Y

PROVIDER: S-EPMC5784765 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Enzyme-Induced Matrix Softening Regulates Hepatocarcinoma Cancer Cell Phenotypes.

Liang Youyun Y Clay Nicholas Edwin NE Sullivan Kathryn M KM Leong Jiayu J Ozcelikkale Altug A Rich Max H MH Lee Min Kyung MK Lai Mei-Hsiu MH Jeon Hojeong H Han Bumsoo B Tong Yen Wah YW Kong Hyunjoon H

Macromolecular bioscience 20170706 9

The progression of cancer is often accompanied by changes in the mechanical properties of an extracellular matrix. However, limited efforts have been made to reproduce these biological events in vitro. To this end, this study demonstrates that matrix remodeling caused by matrix metalloproteinase (MMP)-1 regulates phenotypic activities and modulates radiosensitivity of cancer cells exclusively in a 3D matrix. In this study, hepatocarcinoma cells are cultured in a collagen-based gel tailored to pr ...[more]

PMID: 28683186

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Dataset Information

Enzyme-Induced Matrix Softening Regulates Hepatocarcinoma Cancer Cell Phenotypes.

Publications

Enzyme-Induced Matrix Softening Regulates Hepatocarcinoma Cancer Cell Phenotypes.

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