Ontology highlight
ABSTRACT:
SUBMITTER: Schulz D
PROVIDER: S-EPMC5791659 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Schulz Daniel D Zanotelli Vito Riccardo Tomaso VRT Fischer Jana Raja JR Schapiro Denis D Engler Stefanie S Lun Xiao-Kang XK Jackson Hartland Warren HW Bodenmiller Bernd B
Cell systems 20171227 1
To build comprehensive models of cellular states and interactions in normal and diseased tissue, genetic and proteomic information must be extracted with single-cell and spatial resolution. Here, we extended imaging mass cytometry to enable multiplexed detection of mRNA and proteins in tissues. Three mRNA target species were detected by RNAscope-based metal in situ hybridization with simultaneous antibody detection of 16 proteins. Analysis of 70 breast cancer samples showed that HER2 and CK19 mR ...[more]