Ontology highlight
ABSTRACT:
SUBMITTER: Sun S
PROVIDER: S-EPMC5792436 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Scientific reports 20180131 1
The proofreading activity of the archaeal family B DNA polymerases enables PCR with high fidelity. However, thermostable proofreading DNA polymerases occasionally failed to amplify target fragment that could be amplified by Taq DNA polymerase. We have previously showed that G-rich sequences, which form G-quadruplex, can bind to and inhibit proofreading DNA polymerases. Here we showed that single-stranded oligonucleotides containing sequences of TT(N)mGCCTC can bind and inhibit archaeal family B ...[more]