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TT(N)mGCCTC inhibits archaeal family B DNA polymerases.

ABSTRACT: The proofreading activity of the archaeal family B DNA polymerases enables PCR with high fidelity. However, thermostable proofreading DNA polymerases occasionally failed to amplify target fragment that could be amplified by Taq DNA polymerase. We have previously showed that G-rich sequences, which form G-quadruplex, can bind to and inhibit proofreading DNA polymerases. Here we showed that single-stranded oligonucleotides containing sequences of TT(N)mGCCTC can bind and inhibit archaeal family B DNA polymerases but not Taq DNA polymerase. It is very likely that TT(N)mGCCTC inhibits thermostable DNA polymerases during PCR in a single-stranded form. To the best of our knowledge, this is the first example of DNA sequence that could inhibit DNA polymerase in its single-stranded form.

SUBMITTER: Sun S

PROVIDER: S-EPMC5792436 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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TT(N)mGCCTC inhibits archaeal family B DNA polymerases.

Sun Shuhui S Guo Wei W Yang Jin-Shu JS Qiu Mengsheng M Zhu Xiao-Jing XJ Dai Zhong-Min ZM

Scientific reports 20180131 1

The proofreading activity of the archaeal family B DNA polymerases enables PCR with high fidelity. However, thermostable proofreading DNA polymerases occasionally failed to amplify target fragment that could be amplified by Taq DNA polymerase. We have previously showed that G-rich sequences, which form G-quadruplex, can bind to and inhibit proofreading DNA polymerases. Here we showed that single-stranded oligonucleotides containing sequences of TT(N)mGCCTC can bind and inhibit archaeal family B ...[more]

PMID: 29386523

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