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How water availability influences morphological and biomechanical properties in the one-leaf plant Monophyllaea horsfieldii.

ABSTRACT: In its natural habitat, the one-leaf plant Monophyllaea horsfieldii (Gesneriaceae) shows striking postural changes and dramatic loss of stability in response to intermittently occurring droughts. As the morphological, anatomical and biomechanical bases of these alterations are as yet unclear, we examined the influence of varying water contents on M. horsfieldii by conducting dehydration-rehydration experiments together with various imaging techniques as well as quantitative bending and turgor pressure measurements. As long as only moderate water stress was applied, gradual reductions in hypocotyl diameters and structural bending moduli during dehydration were almost always rapidly recovered in acropetal direction upon rehydration. On an anatomical scale, M. horsfieldii hypocotyls revealed substantial water stress-induced alterations in parenchymatous tissues, whereas the cell form and structure of epidermal and vascular tissues hardly changed. In summary, the functional morphology and biomechanics of M. horsfieldii hypocotyls directly correlated with water status alterations and associated physiological parameters (i.e. turgor pressure). Moreover, M. horsfieldii showed only little passive structural-functional adaptations to dehydration in comparison with poikilohydrous Ramonda myconi.

SUBMITTER: Kampowski T

PROVIDER: S-EPMC5792897 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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How water availability influences morphological and biomechanical properties in the one-leaf plant Monophyllaea horsfieldii.

Kampowski Tim T Mylo Max David MD Poppinga Simon S Speck Thomas T

Royal Society open science 20180103 1

In its natural habitat, the one-leaf plant Monophyllaea horsfieldii (Gesneriaceae) shows striking postural changes and dramatic loss of stability in response to intermittently occurring droughts. As the morphological, anatomical and biomechanical bases of these alterations are as yet unclear, we examined the influence of varying water contents on M. horsfieldii by conducting dehydration-rehydration experiments together with various imaging techniques as well as quantitative bending ...[more]

PMID: 29410820

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Project description:The epiphytic bacterium Pseudomonas syringae strain B728a produces the biosurfactant syringafactin, which is hygroscopic. The water-absorbing potential of syringafactin is high. Syringafactin attracts 250% of its weight in water at high relative humidities but is less hygroscopic at lower relative humidities. This finding suggests that the benefit of syringafactin to the producing cells is strongly context dependent. The contribution of syringafactin to the water availability around cells on different matrices was assessed by examining the water stress exhibited by biosensor strains expressing gfp via the water-stress-activated proU promoter. Wild-type cells exhibited significantly less green fluorescent protein (GFP) fluorescence than a syringafactin-deficient strain on dry filters in atmospheres of high water saturation, as well as on leaf surfaces, indicating greater water availability. When infiltrated into the leaf apoplast, wild-type cells also subsequently exhibited less GFP fluorescence than the syringafactin-deficient strain. These results suggest that the apoplast is a dry but humid environment and that, just as on dry but humid leaf surfaces, syringafactin increases liquid water availability and reduces the water stress experienced by P. syringaeIMPORTANCE Many microorganisms, including the plant pathogen Pseudomonas syringae, produce amphiphilic compounds known as biosurfactants. While biosurfactants are known to disperse hydrophobic compounds and to reduce water tension, they have other properties that can benefit the cells that produce them. Leaf-colonizing bacteria experience frequent water stress, since liquid water is present only transiently on or in leaf sites that they colonize. The demonstration that syringafactin, a biosurfactant produced by P. syringae, is sufficiently hygroscopic to increase water availability to cells, thus relieving water stress, reveals that P. syringae can modify its local habitat both on leaf surfaces and in the leaf apoplast. Such habitat modification may be a common role for biosurfactants produced by other bacterial species that colonize habitats (such as soil) that are not always water saturated.

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How water availability influences morphological and biomechanical properties in the one-leaf plant Monophyllaea horsfieldii.

Publications

How water availability influences morphological and biomechanical properties in the one-leaf plant <i>Monophyllaea horsfieldii</i>.

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