Ontology highlight
ABSTRACT:
SUBMITTER: Sedlak E
PROVIDER: S-EPMC5796215 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
International journal of molecular sciences 20180116 1
<i>Saccharomyces cerevisiae</i> Fet3p is a multicopper oxidase that contains three cupredoxin-like domains and four copper ions located in three distinct metal sites (T1 in domain 3; T2 and the binuclear T3 at the interface between domains 1 and 3). To probe the role of the copper sites in Fet3p thermodynamic stability, we performed urea-induced unfolding experiments with holo-, apo- and three partially-metallated (T1, T2 and T1/T2 sites depleted of copper) forms of Fet3p. Using a combination of ...[more]