Unknown

Dataset Information

0

IL-4 downregulates expression of the target receptor CD30 in neoplastic canine mast cells.


ABSTRACT: CD30 is a novel therapeutic target in human mast cell (MC) neoplasms. In this 'comparative oncology' study, we examined CD30 expression and regulation in neoplastic canine MC using a panel of immunomodulatory cytokines [interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-13 and stem cell factor (SCF)] and the canine mastocytoma cell lines NI-1 and C2. Of all cytokines tested IL-4 was found to downregulate expression of CD30 in NI-1 and C2 cells. We also found that the CD30-targeting antibody-conjugate brentuximab vedotin induces growth inhibition and apoptosis in both MC lines. Next, we asked whether IL-4-induced downregulation of CD30 interferes with brentuximab vedotin-effects. Indeed, pre-incubation of NI-1 cells with IL-4 decreased responsiveness towards brentuximab vedotin. To overcome IL-4-mediated resistance, we applied drug combinations and found that brentuximab vedotin synergizes with the Kit-targeting drugs masitinib and PKC412 in inhibiting growth of NI-1 and C2 cells. In summary, CD30 is a new marker and IL-4-regulated target in neoplastic canine MC.

SUBMITTER: Bauer K 

PROVIDER: S-EPMC5798577 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

IL-4 downregulates expression of the target receptor CD30 in neoplastic canine mast cells.

Bauer K K   Hadzijusufovic E E   Cerny-Reiterer S S   Hoermann G G   Reifinger M M   Pirker A A   Valent P P   Willmann M M  

Veterinary and comparative oncology 20160809 4


CD30 is a novel therapeutic target in human mast cell (MC) neoplasms. In this 'comparative oncology' study, we examined CD30 expression and regulation in neoplastic canine MC using a panel of immunomodulatory cytokines [interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-13 and stem cell factor (SCF)] and the canine mastocytoma cell lines NI-1 and C2. Of all cytokines tested IL-4 was found to downregulate expression of CD30 in NI-1 and C2 cells. We also found that the CD30-targeting antibody-conjugate br  ...[more]

Similar Datasets

| S-EPMC6392733 | biostudies-literature
| S-EPMC7840218 | biostudies-literature
| S-EPMC6900099 | biostudies-literature
| S-EPMC8928232 | biostudies-literature
| S-EPMC3042653 | biostudies-literature
| S-EPMC5037854 | biostudies-literature
| S-EPMC7590161 | biostudies-literature
| S-EPMC3245204 | biostudies-literature
| S-EPMC5559929 | biostudies-other
| S-EPMC3289398 | biostudies-literature