Ontology highlight
ABSTRACT:
SUBMITTER: Najm FJ
PROVIDER: S-EPMC5800952 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Najm Fadi J FJ Strand Christine C Donovan Katherine F KF Hegde Mudra M Sanson Kendall R KR Vaimberg Emma W EW Sullender Meagan E ME Hartenian Ella E Kalani Zohra Z Fusi Nicolo N Listgarten Jennifer J Younger Scott T ST Bernstein Bradley E BE Root David E DE Doench John G JG
Nature biotechnology 20171218 2
Combinatorial genetic screening using CRISPR-Cas9 is a useful approach to uncover redundant genes and to explore complex gene networks. However, current methods suffer from interference between the single-guide RNAs (sgRNAs) and from limited gene targeting activity. To increase the efficiency of combinatorial screening, we employ orthogonal Cas9 enzymes from Staphylococcus aureus and Streptococcus pyogenes. We used machine learning to establish S. aureus Cas9 sgRNA design rules and paired S. aur ...[more]