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GEMINI: a variational Bayesian approach to identify genetic interactions from combinatorial CRISPR screens.

ABSTRACT: Systems for CRISPR-based combinatorial perturbation of two or more genes are emerging as powerful tools for uncovering genetic interactions. However, systematic identification of these relationships is complicated by sample, reagent, and biological variability. We develop a variational Bayes approach (GEMINI) that jointly analyzes all samples and reagents to identify genetic interactions in pairwise knockout screens. The improved accuracy and scalability of GEMINI enables the systematic analysis of combinatorial CRISPR knockout screens, regardless of design and dimension. GEMINI is available as an open source R package on GitHub at https://github.com/sellerslab/gemini .

SUBMITTER: Zamanighomi M

PROVIDER: S-EPMC6624979 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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GEMINI: a variational Bayesian approach to identify genetic interactions from combinatorial CRISPR screens.

Zamanighomi Mahdi M Jain Sidharth S SS Ito Takahiro T Pal Debjani D Daley Timothy P TP Sellers William R WR

Genome biology 20190712 1

Systems for CRISPR-based combinatorial perturbation of two or more genes are emerging as powerful tools for uncovering genetic interactions. However, systematic identification of these relationships is complicated by sample, reagent, and biological variability. We develop a variational Bayes approach (GEMINI) that jointly analyzes all samples and reagents to identify genetic interactions in pairwise knockout screens. The improved accuracy and scalability of GEMINI enables the systematic analysis ...[more]

PMID: 31300006

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