Ontology highlight
ABSTRACT:
SUBMITTER: Tanno H
PROVIDER: S-EPMC7176429 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Tanno Hidetaka H McDaniel Jonathan R JR Stevens Christopher A CA Voss William N WN Li Jie J Durrett Russell R Lee Jiwon J Gollihar Jimmy J Tanno Yuri Y Delidakis George G Pothukuchy Arti A Ellefson Jared W JW Goronzy Jörg J JJ Maynard Jennifer A JA Ellington Andrew D AD Ippolito Gregory C GC Georgiou George G
Science advances 20200422 17
Natively paired sequencing (NPS) of B cell receptors [variable heavy (VH) and light (VL)] and T cell receptors (TCRb and TCRa) is essential for the understanding of adaptive immunity in health and disease. Despite many recent technical advances, determining the VH:VL or TCRb:a repertoire with high accuracy and throughput remains challenging. We discovered that the recently engineered xenopolymerase, RTX, is exceptionally resistant to cell lysate inhibition in single-cell emulsion droplets. We ca ...[more]