Rapid capture and labeling of cells on single domain antibodies-functionalized flow cell.
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ABSTRACT:
SUBMITTER: Chen GY
PROVIDER: S-EPMC5804343 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Rapid capture and labeling of cells on single domain antibodies-functionalized flow cell.
Chen Guan-Yu GY Li Zeyang Z Duarte Joao N JN Esteban Alexandre A Cheloha Ross W RW Theile Christopher S CS Fink Gerald R GR Ploegh Hidde L HL
Biosensors & bioelectronics 20161005 Pt 2
Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. ...[more]
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