Dataset Information

A Mathematical Model for Enzyme Clustering in Glucose Metabolism.

ABSTRACT: We have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular metabolism at subcellular levels has remained challenging. In this work, we develop a mathematical model using a system of ordinary differential equations, in which the association of the rate-limiting enzymes into multienzyme complexes is included as an essential element. We then demonstrate that our mathematical model provides a quantitative principle to simulate glucose flux at both subcellular and population levels in human cancer cells. Lastly, we use the model to simulate 2-deoxyglucose-mediated alteration of glucose flux in a population level based on subcellular high-content imaging data. Collectively, we introduce a new mathematical model for human glucose metabolism, which promotes our understanding of functional roles of differently sized multienzyme complexes in both single-cell and population levels.

SUBMITTER: Jeon M

PROVIDER: S-EPMC5807315 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Publications

A Mathematical Model for Enzyme Clustering in Glucose Metabolism.

Jeon Miji M Kang Hye-Won HW An Songon S

Scientific reports 20180209 1

We have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular ...[more]

PMID: 29426820

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Dataset Information

A Mathematical Model for Enzyme Clustering in Glucose Metabolism.

Publications

A Mathematical Model for Enzyme Clustering in Glucose Metabolism.

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