CellCycleTRACER accounts for cell cycle and volume in mass cytometry data.
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ABSTRACT:
SUBMITTER: Rapsomaniki MA
PROVIDER: S-EPMC5809393 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Publications
CellCycleTRACER accounts for cell cycle and volume in mass cytometry data.
Rapsomaniki Maria Anna MA Lun Xiao-Kang XK Woerner Stefan S Laumanns Marco M Bodenmiller Bernd B Martínez María Rodríguez MR
Nature communications 20180212 1
Recent studies have shown that cell cycle and cell volume are confounding factors when studying biological phenomena in single cells. Here we present a combined experimental and computational method, CellCycleTRACER, to account for these factors in mass cytometry data. CellCycleTRACER is applied to mass cytometry data collected on three different cell types during a TNFα stimulation time-course. CellCycleTRACER reveals signaling relationships and cell heterogeneity that were otherwise masked. ...[more]
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