Soil microbiome transfer method affects microbiome composition, including dominant microorganisms, in a novel environment.
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ABSTRACT:
SUBMITTER: Howard MM
PROVIDER: S-EPMC5812521 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
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Soil microbiome transfer method affects microbiome composition, including dominant microorganisms, in a novel environment.
Howard Mia M MM Bell Terrence H TH Kao-Kniffin Jenny J
FEMS microbiology letters 20170601 11
We show that choice of soil microbiome transfer method, i.e. direct soil transfers and a common soil wash procedure, dramatically influences the microbiome that develops in a new environment, using high-throughput amplicon sequencing of 16S rRNA genes and the fungal internal transcribed spacer (ITS) region. After 3 weeks of incubation in commercial potting mix, microbiomes were most similar to the source soil when a greater volume of initial soil was transferred (5% v/v transfer), and least simi ...[more]
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