Inhibition of NF-?B prevents the acidic bile-induced oncogenic mRNA phenotype, in human hypopharyngeal cells.

ABSTRACT: Bile-containing gastro-duodenal reflux has been clinically considered an independent risk factor in hypopharyngeal carcinogenesis. We recently showed that the chronic effect of acidic bile, at pH 4.0, selectively induces NF-?B activation and accelerates the transcriptional levels of genes, linked to head and neck cancer, in normal hypopharyngeal epithelial cells. Here, we hypothesize that NF-?B inhibition is capable of preventing the acidic bile-induced and cancer-related mRNA phenotype, in treated normal human hypopharyngeal cells. In this setting we used BAY 11-7082, a specific and well documented pharmacologic inhibitor of NF-?B, and we observed that BAY 11-7082 effectively inhibits the acidic bile-induced gene expression profiling of the NF-?B signaling pathway (down-regulation of 72 out of 84 analyzed genes). NF-?B inhibition significantly prevents the acidic bile-induced transcriptional activation of NF-?B transcriptional factors, RELA (p65) and c-REL, as well as genes related to and commonly found in established HNSCC cell lines. These include anti-apoptotic bcl-2, oncogenic STAT3, EGFR, ?Np63, TNF-? and WNT5A, as well as cytokines IL-1? and IL-6. Our findings are consistent with our hypothesis demonstrating that NF-?B inhibition effectively prevents the acidic bile-induced cancer-related mRNA phenotype in normal human hypopharyngeal epithelial cells supporting an understanding that NF-?B may be a critical link between acidic bile and early preneoplastic events in this setting.

SUBMITTER: Vageli DP 

PROVIDER: S-EPMC5814181 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature