Ontology highlight
ABSTRACT:
SUBMITTER: Keeble AH
PROVIDER: S-EPMC5814910 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Keeble Anthony H AH Banerjee Anusuya A Ferla Matteo P MP Reddington Samuel C SC Anuar Irsyad N A Khairil INAK Howarth Mark M
Angewandte Chemie (International ed. in English) 20171205 52
SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures. We initially developed SpyTag and SpyCatcher by rational design, through splitting a domain from a Gram-positive bacterial adhesin. In this work, we established a phage-display platform to select for specific amidation, leading to an or ...[more]