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Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome.


ABSTRACT: Proper chromosome segregation is essential in all living organisms. In Caulobacter crescentus, the ParA-ParB-parS system is required for proper chromosome segregation and cell viability. The bacterial centromere-like parS DNA locus is the first to be segregated following chromosome replication. parS is bound by ParB protein, which in turn interacts with ParA to partition the ParB-parS nucleoprotein complex to each daughter cell. Here, we investigated the genome-wide distribution of ParB on the Caulobacter chromosome using a combination of in vivo chromatin immunoprecipitation (ChIP-seq) and in vitro DNA affinity purification with deep sequencing (IDAP-seq). We confirmed two previously identified parS sites and discovered at least three more sites that cluster ?8 kb from the origin of replication. We showed that Caulobacter ParB nucleates at parS sites and associates non-specifically with ?10 kb flanking DNA to form a high-order nucleoprotein complex on the left chromosomal arm. Lastly, using transposon mutagenesis coupled with deep sequencing (Tn-seq), we identified a ?500 kb region surrounding the native parS cluster that is tolerable to the insertion of a second parS cluster without severely affecting cell viability. Our results demonstrate that the genomic distribution of parS sites is highly restricted and is crucial for chromosome segregation in Caulobacter.

SUBMITTER: Tran NT 

PROVIDER: S-EPMC5815017 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome.

Tran Ngat T NT   Stevenson Clare E CE   Som Nicolle F NF   Thanapipatsiri Anyarat A   Jalal Adam S B ASB   Le Tung B K TBK  

Nucleic acids research 20180201 3


Proper chromosome segregation is essential in all living organisms. In Caulobacter crescentus, the ParA-ParB-parS system is required for proper chromosome segregation and cell viability. The bacterial centromere-like parS DNA locus is the first to be segregated following chromosome replication. parS is bound by ParB protein, which in turn interacts with ParA to partition the ParB-parS nucleoprotein complex to each daughter cell. Here, we investigated the genome-wide distribution of ParB on the C  ...[more]

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