Dataset Information

T cells responding to Trypanosoma cruzi detected by membrane TNF-? and CD154 in chagasic patients.

ABSTRACT:

Introduction

Chagas disease is a parasitic infection whose pathogenesis is related to parasite persistence and a dysfunctional cellular immune response. Variability in cytokine secretion among chronic Trypanosoma cruzi-infected patients might preclude the identification of the pool of antigen specific T cells. The goal of this study was to determine the fraction of T cells responding to T. cruzi antigen measured by the expression of membrane TNF-? and CD154.

Methods

A total of 21 chagasic patients, 11 healthy and 5 non-chagasic cardiomyopathy controls were analyzed. PBMCs were short-term cultured in the presence of anti-CD28, anti-CD49d, anti-TNF-?, and TACE (TNF-? converting enzyme) inhibitor either under T. cruzi-lysate or polyclonal stimuli. Cells were stained with anti-CD3, anti-CD4, anti-CD8, and anti-CD154, and analyzed with flow cytometry.

Results

CD4+ and CD8+ T cells in chagasic patients displayed higher percentages of membrane-bound TNF-?+ and CD154+ compared with controls after T. cruzi-antigen stimulation. Both markers displayed a positive correlation in the T cell subpopulations analyzed. Symptomatic chagasic patients were differentiated from asymptomatic patients based on the expression of CD154 and membrane TNF-? in TCD4+ and TCD8+ compartments, respectively.

Conclusions

These results show that both markers could be useful for assessing the pool of antigen-specific T cells in chronic chagasic patients.

SUBMITTER: Ripoll JG

PROVIDER: S-EPMC5818450 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Publications

T cells responding to Trypanosoma cruzi detected by membrane TNF-α and CD154 in chagasic patients.

Ripoll Juan G JG Giraldo Nicolás A NA Bolaños Natalia I NI Roa Nubia N Rosas Fernando F Cuéllar Adriana A Puerta Concepción J CJ González John M JM

Immunity, inflammation and disease 20171001 1

<h4>Introduction</h4>Chagas disease is a parasitic infection whose pathogenesis is related to parasite persistence and a dysfunctional cellular immune response. Variability in cytokine secretion among chronic Trypanosoma cruzi-infected patients might preclude the identification of the pool of antigen specific T cells. The goal of this study was to determine the fraction of T cells responding to T. cruzi antigen measured by the expression of membrane TNF-α and CD154.<h4>Methods</h4>A total of 21 ...[more]

PMID: 28967229

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