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LINE-1 protein localization and functional dynamics during the cell cycle.

ABSTRACT: LINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology. However, a clear understanding of L1's lifecycle and the processes involved in restricting its insertion and intragenomic spread remains elusive. Here we identify modes of L1 proteins' entrance into the nucleus, a necessary step for L1 proliferation. Using functional, biochemical, and imaging approaches, we also show a clear cell cycle bias for L1 retrotransposition that peaks during the S phase. Our observations provide a basis for novel interpretations about the nature of nuclear and cytoplasmic L1 ribonucleoproteins (RNPs) and the potential role of DNA replication in L1 retrotransposition.

SUBMITTER: Mita P

PROVIDER: S-EPMC5821460 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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LINE-1 protein localization and functional dynamics during the cell cycle.

Mita Paolo P Wudzinska Aleksandra A Sun Xiaoji X Andrade Joshua J Nayak Shruti S Kahler David J DJ Badri Sana S LaCava John J Ueberheide Beatrix B Yun Chi Y CY Fenyö David D Boeke Jef D JD

eLife 20180108

LINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology. However, a clear understanding of L1's lifecycle and the processes involved in restricting its insertion and intragenomic spread remains elusive. Here we identify modes of L1 proteins' entr ...[more]

PMID: 29309036

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