A map of protein dynamics during cell-cycle progression and cell-cycle exit

ABSTRACT: The cell-cycle field has identified the core regulators that drive the cell cycle, but we do not have a clear map of the dynamics of these regulators during cell-cycle progression vs. cell-cycle exit. Here we use single-cell time-lapse microscopy of Cyclin-Dependent Kinase 2 (CDK2) activity followed by end-point immunofluorescence and computational cell synchronization to determine the temporal dynamics of key cell-cycle proteins in asynchronously cycling human cells. We identify several unexpected patterns for core cell-cycle proteins in actively proliferating (CDK2-increasing) vs. spontaneously quiescent (CDK2-low) cells, including Cyclin D1, whose levels we find to be higher in spontaneously quiescent vs. proliferating cells. We also identify proteins whose concentrations steadily inc

SUBMITTER: Mingwei Min 

PROVIDER: S-BSST21 | biostudies-other |

REPOSITORIES: biostudies-other