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Reflectance confocal microscopy of oral epithelial tissue using an electrically tunable lens.

ABSTRACT: We present the use of a commercially available electrically tunable lens to achieve axial scanning in a reflectance confocal microscope. Over a 255 ?m axial scan range, the lateral and axial resolutions varied from 1-2 ?m and 4-14 ?m, respectively, dependent on the variable focal length of the tunable lens. Confocal imaging was performed on normal human biopsies from the oral cavity ex vivo. Sub-cellular morphologic features were seen throughout the depth of the epithelium while axially scanning using the focus tunable lens.

SUBMITTER: Jabbour JM 

PROVIDER: S-EPMC5830149 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Reflectance confocal microscopy of oral epithelial tissue using an electrically tunable lens.

Jabbour Joey M JM   Malik Bilal H BH   Cuenca Rodrigo R   Cheng Shuna S   Jo Javier A JA   Cheng Yi-Shing L YL   Wright John M JM   Maitland Kristen C KC  

Proceedings of SPIE--the International Society for Optical Engineering 20140227

We present the use of a commercially available electrically tunable lens to achieve axial scanning in a reflectance confocal microscope. Over a 255 μm axial scan range, the lateral and axial resolutions varied from 1-2 μm and 4-14 μm, respectively, dependent on the variable focal length of the tunable lens. Confocal imaging was performed on normal human biopsies from the oral cavity ex vivo. Sub-cellular morphologic features were seen throughout the depth of the epithelium while axially scanning  ...[more]

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