Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Optical axial scanning in confocal microscopy using an electrically tunable lens.

ABSTRACT: This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial tissue and compared to traditional stage scanning. By enabling rapid axial scanning, minimizing motion artifacts, and reducing mechanical complexity, this technique has potential to enhance in vivo three-dimensional imaging in confocal endomicroscopy.

SUBMITTER: Jabbour JM

PROVIDER: S-EPMC3920893 | biostudies-other | 2014 Feb

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Reflectance confocal microscopy of oral epithelial tissue using an electrically tunable lens.

Project description:We present the use of a commercially available electrically tunable lens to achieve axial scanning in a reflectance confocal microscope. Over a 255 ?m axial scan range, the lateral and axial resolutions varied from 1-2 ?m and 4-14 ?m, respectively, dependent on the variable focal length of the tunable lens. Confocal imaging was performed on normal human biopsies from the oral cavity ex vivo. Sub-cellular morphologic features were seen throughout the depth of the epithelium while axially scanning using the focus tunable lens.

| S-EPMC5830149 | biostudies-literature

Remote focal scanning optical projection tomography with an electrically tunable lens.

Project description:We describe a remote focal scanning technique for optical projection tomography (OPT) implemented with an electrically tunable lens (ETL) that removes the need to scan the specimen or objective lens. Using a 4× objective lens the average spatial resolution is improved by ∼46% and the light collection efficiency by a factor of ∼6.76, thereby enabling increased acquisition speed and reduced light dose. This convenient implementation is particularly appropriate for lower magnifications and larger sample diameters where axial objective scanning would encounter problems with speed and stability.

| S-EPMC4206308 | biostudies-other

Confocal laser scanning microscopy for rapid optical characterization of graphene.

Project description:Two-dimensional (2D) materials such as graphene have become the focus of extensive research efforts in condensed matter physics. They provide opportunities for both fundamental research and applications across a wide range of industries. Ideally, characterization of graphene requires non-invasive techniques with single-atomic-layer thickness resolution and nanometer lateral resolution. Moreover, commercial application of graphene requires fast and large-area scanning capability. We demonstrate the optimized balance of image resolution and acquisition time of non-invasive confocal laser scanning microscopy (CLSM), rendering it an indispensable tool for rapid analysis of mass-produced graphene. It is powerful for analysis of 1-5 layers of exfoliated graphene on Si/SiO2, and allows us to distinguish the interfacial layer and 1-3 layers of epitaxial graphene on SiC substrates. Furthermore, CLSM shows excellent correlation with conventional optical microscopy, atomic force microscopy, Kelvin probe force microscopy, conductive atomic force microscopy, scanning electron microscopy and Raman mapping.

| S-EPMC6512973 | biostudies-literature

Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus tunable lens.

Project description:Self-interference digital holographic microscopy (DHM) has been found particular suitable for simplified quantitative phase imaging of living cells. However, a main drawback of the self-interference DHM principle are scattering patterns that are induced by the coherent nature of the laser light which affect the resolution for detection of optical path length changes. We present a simple and efficient technique for the reduction of coherent disturbances in quantitative phase images. Therefore, amplitude and phase of the sample illumination are modulated by an electrically focus tunable lens. The proposed method is in particular convenient with the self-interference DHM concept. Results from the characterization of the method show that a reduction of coherence induced disturbances up to 70 percent can be achieved. Finally, the performance for enhanced quantitative imaging of living cells is demonstrated.

| S-EPMC4285600 | biostudies-other

Axial plane optical microscopy.

Project description:We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.

| S-EPMC4248283 | biostudies-literature

Scanning Electrochemical Microscopy of Electrically Heated Wire Substrates.

Project description:We report a new configuration for enhancing the performance of scanning electrochemical microscopy (SECM) via heating of the substrate electrode. A flattened Pt microwire was employed as the substrate electrode. The substrate was heated by an alternating current (AC), resulting in an increased mass transfer between the wire surface and the bulk solution. The electrochemical response of the Pt wire during heating was investigated by means of cyclic voltammetry (CV). The open circuit potential (OCP) of the wire was recorded over time, while varied heating currents were applied to investigate the time needed for establishing steady-state conditions. Diffusion layer studies were carried out by performing probe approach curves (PACs) for various measuring modes of SECM. Finally, imaging studies of a heated substrate electrode surface, applying feedback, substrate generation/tip collection (SG/TC), and the competition mode of SECM, were performed and compared with room temperature results.

| S-EPMC7179101 | biostudies-literature

Electrically Tunable Optical Metasurfaces for Dynamic Polarization Conversion.

Project description:Dynamic control over the polarization of light is highly desirable in many optical applications, including optical communications, laser science, three-dimensional displays, among others. Conventional methods for polarization control are often based on bulky optical elements. To achieve highly integrated optical devices, metasurfaces, which have been intensively studied in recent years, hold great promises to replace conventional optical elements for a variety of optical functions. In this work, we demonstrate electrically tunable optical metasurfaces for dynamic polarization conversion at visible frequencies. By exploring both the geometric and propagation phase tuning capabilities, rapid and reversible polarization rotation up to 90° is achieved for linearly polarized light. The dynamic functionality is imparted by liquid crystals, which serve as a thin surrounding medium with electrically tunable refractive indices for the metasurface antennas. Furthermore, we expand our concept to demonstrate electrically tunable metasurfaces for dynamic holography and holographic information generation with independently controlled multiple pixels.

| S-EPMC8361430 | biostudies-literature

Reflectance confocal endomicroscope with optical axial scanning for in vivo imaging of the oral mucosa.

Project description:This paper presents the design and evaluation of a reflectance confocal laser endomicroscope using a miniature objective lens within a rigid probe in conjunction with an electrically tunable lens for axial scanning. The miniature lens was characterized alone as well as in the endoscope across a 200 µm axial scan range using the tunable lens. The ability of the confocal endoscope to probe the human oral cavity is demonstrated by imaging of the oral mucosa in vivo. The results indicate that reflectance confocal endomicroscopy has the potential to be used in a clinical setting and guide diagnostic evaluation of biological tissue.

| S-EPMC4242017 | biostudies-other

Speeded-Up Focus Control of Electrically Tunable Lens by Sparse Optimization.

Project description:Electrically tunable lenses (ETL), also known as liquid lenses, can be focused at various distances by changing the electric signal applied on the lens. ETLs require no mechanical structures, and therefore, provide a more compact and inexpensive focus control than conventional computerized translation stages. They have been exploited in a wide range of imaging and display systems and enabled novel applications for the last several years. However, the optical fluid in the ETL is rippled after the actuation, which physically limits the response time and significantly hampers the applicability range. To alleviate this problem, we apply a sparse optimization framework that optimizes the temporal pattern of the electrical signal input to the ETL. In verification experiments, the proposed method accelerated the convergence of the focal length to the target patterns. In particular, it converged the optical power to the target at twice the speed of the simply determined input signal, and increased the quality of the captured image during multi-focal imaging.

| S-EPMC6710262 | biostudies-literature

Quantitative single-molecule imaging by confocal laser scanning microscopy.

Project description:A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct determination of the number of fluorescent molecules and their diffusion rates without resorting to temporal or spatial autocorrelation analyses. Digital images of fluorescent molecules were recorded by using fast scanning and avalanche photodiode detectors. In this way the signal-to-background ratio was significantly improved, enabling direct quantitative imaging by CLSM. The potential of the proposed approach is demonstrated by using standard solutions of fluorescent dyes, fluorescently labeled DNA molecules, quantum dots, and the Enhanced Green Fluorescent Protein in solution and in live cells. The method was verified by using fluorescence correlation spectroscopy. The relevance for biological applications, in particular, for live cell imaging, is discussed.

| S-EPMC2587633 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data