The circadian regulator BMAL1 programmes responses to parasitic worm infection via a dendritic cell clock.

ABSTRACT: Resistance to the intestinal parasitic helminth Trichuris muris requires T-helper 2 (T_H2) cellular and associated IgG1 responses, with expulsion typically taking up to 4 weeks in mice. Here, we show that the time-of-day of the initial infection affects efficiency of worm expulsion, with strong T_H2 bias and early expulsion in morning-infected mice. Conversely, mice infected at the start of the night show delayed resistance to infection, and this is associated with feeding-driven metabolic cues, such that feeding restriction to the day-time in normally nocturnal-feeding mice disrupts parasitic expulsion kinetics. We deleted the circadian regulator BMAL1 in antigen-presenting dendritic cells (DCs) in vivo and found a loss of time-of-day dependency of helminth expulsion. RNAseq analyses revealed that IL-12 responses to worm antigen by circadian-synchronised DCs were dependent on BMAL1. Therefore, we find that circadian machinery in DCs contributes to the T_H1/T_H2 balance, and that environmental, or genetic perturbation of the DC clock results in altered parasite expulsion kinetics.

SUBMITTER: Hopwood TW 

PROVIDER: S-EPMC5830501 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature