Dataset Information

Fluorescent component and complexation mechanism of extracellular polymeric substances during dye wastewater biotreatment by anaerobic granular sludge.

ABSTRACT: In this study, methylene blue (MB) wastewater was biotreated by anaerobic granular sludge (AnGS), and the fluorescent components of extracellular polymeric substances (EPS) and complexation mechanism were evaluated. Based on the experimental data, the sorption of MB by both live and inactivated AnGS followed the pseudo-second-order model, and the adsorption isotherm conformed well to the Langmuir model. It was shown that the difference in the sorption of live and inactivated AnGS was not significant, indicating that the sorption is mainly a physical-chemical process and metabolically mediated diffusion is negligible. The interaction between EPS and MB was proved by three-dimensional excitation-emission matrix (3D-EEM) and synchronous fluorescence spectra. 3D-EEM indicated that protein (PN)-like substances were the main peaks of EPS, and gradually quenched with increase of MB concentrations. According to synchronous fluorescence spectra, the main fluorescence quenching was caused by PN-like and humic-like fractions, and belonged to the static type of quenching. FTIR spectra demonstrated that hydroxyl and amino groups played a major role in MB sorption.

SUBMITTER: Li N

PROVIDER: S-EPMC5830752 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Fluorescent component and complexation mechanism of extracellular polymeric substances during dye wastewater biotreatment by anaerobic granular sludge.

Li Na N Wei Dong D Sun Qunqun Q Han Xiao X Du Bin B Wei Qin Q

Royal Society open science 20180228 2

In this study, methylene blue (MB) wastewater was biotreated by anaerobic granular sludge (AnGS), and the fluorescent components of extracellular polymeric substances (EPS) and complexation mechanism were evaluated. Based on the experimental data, the sorption of MB by both live and inactivated AnGS followed the pseudo-second-order model, and the adsorption isotherm conformed well to the Langmuir model. It was shown that the difference in the sorption of live and inactivated AnGS was not signifi ...[more]

PMID: 29515863

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Project description:Wastewater treatment plants (WWTP) are regarded as the last barriers for the release of incompletely separated and recycled nanoparticles (NPs) into the environment. Despite the importance and ubiquity of microbial extracellular polymeric substances (EPSs) in the complex wastewater matrix, the interaction between NPs and EPSs of anaerobic microflora involved in wastewater treatment and the resultant impact on the biomass metabolomics are unclear. Thus, the impacts of different metal oxide (TiO2, ZnO, and CuO) NPs on functional bacteria in anaerobic granular sludge (AGS) and the possible toxicity mechanisms were investigated. In particular, the binding quality, enhanced resistance mechanism, and chemical fractional contribution of EPSs from AGS against the nanotoxicity of different NPs was assessed. The results showed that CuO NPs caused the most severe inhibition towards acetoclastic and hydrogenotrophic methanogens, followed by ZnO NPs, whereas TiO2 NPs caused no inhibition to methanogenesis. Excessive EPS production, especially the protein-like substances, was an effective strategy for reducing certain NPs' toxicity by immobilizing NPs away from AGS cells, whereas the metabolism restriction on inner microorganisms of AGS induced by CuO NPs can deteriorate the protective role of EPS, indicating that the roles of EPS may not be amenable to generalizations. Further investigations with lactate dehydrogenase (LDH) and reactive oxygen species (ROS) assays indicated that there are greatly essential differences between the toxicity mechanisms of metal NPs to AGS, which varied depending on the NPs' type and dosage. In addition, dynamic changes in the responses of EPS content to different NPs can result in a significant shift in methanogenic and acidogenic microbial communities. Thus, the production and composition of EPSs will be a key factor in determining the fate and potential effect of NPs in the complex biological matrix. In conclusion, this study broadens the understanding of the inhibition mechanisms of metal oxide NPs on the AGS process, and the influence of EPSs on the fate, behavior, and toxicity of NPs.

Dataset Information

Fluorescent component and complexation mechanism of extracellular polymeric substances during dye wastewater biotreatment by anaerobic granular sludge.

Publications

Fluorescent component and complexation mechanism of extracellular polymeric substances during dye wastewater biotreatment by anaerobic granular sludge.

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