Dataset Information

Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge.

ABSTRACT: Phosphorus (P) is an essential element for living organisms and anaerobic sludge is an attractive source for P recovery. Anaerobic P release depends on both phosphorus-accumulating organisms (PAOs) and extracellular polymeric substances (EPS). However, the P release contributed by the microbial cells and EPS was not addressed completely and the effect of temperature on the mechanism of P release and transformation was rarely considered. This study, therefore, investigated the effects of temperature on the P fraction and the relationship between PAOs metabolic pathway and EPS reaction using the Standards in Measurements and Testing (SMT) protocol and the ³¹P nuclear magnetic resonance (³¹P-NMR) experiments. Experimental results showed that the temperature not only affected the metabolism of PAOs, but also significantly influenced the EPS components and the hydrolysis of EPS-associated polyphosphate (poly-P). And the P release mainly occurred due to biological mechanisms with a conversion from non-reactive P (NRP) in both intracellular and extracellular substances to reactive P (RP) fractions. The highest concentration of total P in the supernatant (TP_L) occurred at 15 °C, and the TP_L release from the solid to liquid phase was better fitted with pseudo-second-order kinetic model. More organic P in the sludge (OP_s) released from the sludge phase at 35 °C would convert into inorganic P (IP_s) and non-apatite inorganic phosphorus (NAIP_s) was the most labile P fraction for P release. The hydrolysis of EPS-associated poly-P was enhanced by higher temperatures with the degradation of the long-chain poly-P by PAOs. Meanwhile, a lower temperature could obviously improve the P release because the dominance of PAOs would potentially shift to GAOs with the increase of temperature. But the very-low temperature (5 °C) was not beneficial for the P release and suppressed the microbial activities.

SUBMITTER: Zeng F

PROVIDER: S-EPMC9059762 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge.

Zeng Fanzhe F Jin Wenbiao W Zhao Qingliang Q Zhao Qingliang Q

RSC advances 20190116 4

Phosphorus (P) is an essential element for living organisms and anaerobic sludge is an attractive source for P recovery. Anaerobic P release depends on both phosphorus-accumulating organisms (PAOs) and extracellular polymeric substances (EPS). However, the P release contributed by the microbial cells and EPS was not addressed completely and the effect of temperature on the mechanism of P release and transformation was rarely considered. This study, therefore, investigated the effects of temperat ...[more]

PMID: 35516154

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Project description:Wastewater treatment plants (WWTP) are regarded as the last barriers for the release of incompletely separated and recycled nanoparticles (NPs) into the environment. Despite the importance and ubiquity of microbial extracellular polymeric substances (EPSs) in the complex wastewater matrix, the interaction between NPs and EPSs of anaerobic microflora involved in wastewater treatment and the resultant impact on the biomass metabolomics are unclear. Thus, the impacts of different metal oxide (TiO2, ZnO, and CuO) NPs on functional bacteria in anaerobic granular sludge (AGS) and the possible toxicity mechanisms were investigated. In particular, the binding quality, enhanced resistance mechanism, and chemical fractional contribution of EPSs from AGS against the nanotoxicity of different NPs was assessed. The results showed that CuO NPs caused the most severe inhibition towards acetoclastic and hydrogenotrophic methanogens, followed by ZnO NPs, whereas TiO2 NPs caused no inhibition to methanogenesis. Excessive EPS production, especially the protein-like substances, was an effective strategy for reducing certain NPs' toxicity by immobilizing NPs away from AGS cells, whereas the metabolism restriction on inner microorganisms of AGS induced by CuO NPs can deteriorate the protective role of EPS, indicating that the roles of EPS may not be amenable to generalizations. Further investigations with lactate dehydrogenase (LDH) and reactive oxygen species (ROS) assays indicated that there are greatly essential differences between the toxicity mechanisms of metal NPs to AGS, which varied depending on the NPs' type and dosage. In addition, dynamic changes in the responses of EPS content to different NPs can result in a significant shift in methanogenic and acidogenic microbial communities. Thus, the production and composition of EPSs will be a key factor in determining the fate and potential effect of NPs in the complex biological matrix. In conclusion, this study broadens the understanding of the inhibition mechanisms of metal oxide NPs on the AGS process, and the influence of EPSs on the fate, behavior, and toxicity of NPs.

Dataset Information

Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge.

Publications

Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge.

Similar Datasets

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