Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MnO2 Nanotube-Based NanoSearchlight for Imaging of Multiple MicroRNAs in Live Cells.

ABSTRACT: Sensitive assay and imaging of multiple low-abundance microRNAs (miRNAs) in living cells remain a grand challenge. Herein, based on polyelectrolyte-induced reduction, a facile approach has been proposed to synthesize novel MnO2 nanotubes. Owing to the remarkably strong fluorescence quenching ability, low cytotoxicity, and excellent colloid stability, the as-prepared MnO2 nanotubes showed great potential for simultaneous detection and imaging of multiple miRNAs in vitro and in situ in living cells for the first time. Besides, MnO2 nanotubes can be reduced to Mn2+ by intracellular acid pH or glutathione, which may serve as an activatable contrast reagent for MRI. Therefore, the MnO2 nanotube-based probes, termed "NanoSearchlight", provide a promising, multimodal imaging tool for precise and accurate diagnosis and prognosis of cancers.

SUBMITTER: Lu Q

PROVIDER: S-EPMC5831178 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

MnO2 Nanotube-Based NanoSearchlight for Imaging of Multiple MicroRNAs in Live Cells.

Lu Qian Q Ericson Daniel D Song Yang Y Zhu Chengzhou C Ye Ranfeng R Liu Songqin S Spernyak Joseph A JA Du Dan D Li He H Wu Yun Y Lin Yuehe Y

ACS applied materials & interfaces 20170703 28

Sensitive assay and imaging of multiple low-abundance microRNAs (miRNAs) in living cells remain a grand challenge. Herein, based on polyelectrolyte-induced reduction, a facile approach has been proposed to synthesize novel MnO2 nanotubes. Owing to the remarkably strong fluorescence quenching ability, low cytotoxicity, and excellent colloid stability, the as-prepared MnO2 nanotubes showed great potential for simultaneous detection and imaging of multiple miRNAs in vitro and ...[more]

PMID: 28493665

Similar Datasets

Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells.

Project description:MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells. The nanoflares consist of gold nanoparticles (AuNPs) functionalized with a dense shell of recognition sequences hybridized to two short fluorophore-labeled DNA molecules, termed "flares". In this conformation, the close proximity of the fluorophore to the AuNPs surface leads to quenching of the fluorescence. However, when target miRNAs bind to the recognition sequence, the concomitant displacement of the flare can be detected as a corresponding increase in fluorescence. The results demonstrate that the two-color-based nanoflares can simultaneously detect miR-21 and miR-141 expression levels in various live cancer cells successfully. Compared to the traditional single-color-based nanoflares, the two-color-based nanoflares could offer more reliable and practical information for cancer detection, improving the accuracy of early disease diagnosis.

| S-EPMC5743841 | biostudies-other

Electrodeposition of α-MnO2/γ-MnO2 on Carbon Nanotube for Yarn Supercapacitor.

Project description:Yarn supercapacitors have attracted renewed interest as promising energy storage for wearable devices due to their lightweight, long cycling lifetime and excellent weavability. There has been much effort to fabricate high performance yarn supercapacitor by depositing pseudo-capacitive materials on the outer surface of the carbon fibers. However, a key challenge still remains to achieve high capacitance and high mass loading without sacrificing the cycling stability. Herein, we perform a phase-controlled of MnO2 at various deposition temperatures with ultrahigh mass loading of 11 mg/cm2 on a MWNT sheets and fabricate it to yarn structure to achieve high capacitance without decreasing in the electrochemical performance. The structure of optimized sample (MnO2/CNTs-60, deposition at 60 °C) consists of the composite of primary α-MnO2 nanosheets and secondary γ-MnO2 nanoparticles. The heteronanostructures of MnO2 provide facile ionic and electric transport in the yarn electrode, resulting in improvement of electrochemical performance and cycling stability. The MnO2/CNTs-60 yarn electrode with ultrahigh mass loading delivers a high areal capacitance of 3.54 F/cm2 at 1 mA/cm2 and an excellent rate capability. Finally, the MnO2/CNTs-60 device exhibits an outstanding high areal energy density of 93.8 μWh/cm2 at the power density of 193 μW/cm2, which is superior to previously reported symmetric yarn supercapacitors.

| S-EPMC6677808 | biostudies-literature

Measuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells.

Project description:Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation states of nanotubes transiently as well as the cell position, denoted by lipid and protein signals. This work portends the real-time molecular imaging of live cells and tissues using Raman spectroscopy, affording multiplexing and complete photostability.

| S-EPMC3561480 | biostudies-literature

Bubble-propelled micromotors based on hierarchical MnO2 wrapped carbon nanotube aggregates for dynamic removal of pollutants.

Project description:Water pollution is currently an urgent public health and environmental issue. Bubble-propelled micromotors might offer an effective approach for dealing with environmental contamination. Herein, we present the synthesis of multi-walled carbon nanotube (MWCNT)/manganese dioxide (MnO2) micromotors based on MWCNT aggregates as microscale templates by a simple one-step hydrothermal procedure. The morphology, composition, and structure of the obtained MWCNT/MnO2 micromotors were characterized in detail. The MnO2 nanoflakes formed a catalytic layer on the MWCNT backbone, which promoted effective bubble evolution and propulsion at remarkable speeds of 359.31 μm s-1. The bubble velocity could be modulated based on the loading of MnO2 nanoflakes. The rapid movement of these MWCNT/MnO2 catalytic micromotors resulted in a highly efficient moving adsorption platform, which considerably enhanced the effectiveness of water purification. Dynamic adsorption of organic dyes by the micromotors increased the degradation rate to approximately 4.8 times as high as that of their corresponding static counterparts. The adsorption isotherms and adsorption kinetics were also explored. The adsorption mechanism was well fitted by the Langmuir model, following pseudo-second-order kinetics. Thus, chemisorption of Congo red at the heterogeneous MnO2 wrapped microimotor surface was the rate determining step. The high propulsion speed and remarkable decontamination efficiency of the MWCNT/MnO2 micromotors indicate potential for environmental contamination applications.

| S-EPMC9052125 | biostudies-literature

MnO2 nanosheets as a carrier and accelerator for improved live-cell biosensing application of CRISPR/Cas12a.

Project description:Besides gene-editing, the CRISPR/Cas12a system has also been widely used in in vitro biosensing, but its applications in live-cell biosensing are rare. One reason is lacking appropriate carriers to synchronously deliver all components of the CRISPR/Cas12a system into living cells. Herein, we demonstrate that MnO2 nanosheets are an excellent carrier of CRISPR/Cas12a due to the two important roles played by them. Through a simple mixing operation, all components of the CRISPR/Cas12a system can be loaded on MnO2 nanosheets and thus synchronously delivered into cells. Intracellular glutathione (GSH)-induced decomposition of MnO2 nanosheets not only results in the rapid release of the CRISPR/Cas12a system in cells but also provides Mn2+ as an accelerator to promote CRISPR/Cas12a-based biosensing of intracellular targets. Due to the merits of highly efficient delivery, rapid intracellular release, and the accelerated signal output reaction, MnO2 nanosheets work better than commercial liposome carriers in live-cell biosensing analysis of survivin messenger RNA (mRNA), producing much brighter fluorescence images in a shorter time. The use of MnO2 nanosheets might provide a good carrier for different CRISPR/Cas systems and achieve the rapid and sensitive live-cell biosensing analysis of different intracellular targets, thus paving a promising way to promote the applications of CRISPR/Cas systems in living cells.

| S-EPMC9007066 | biostudies-literature

Construction of ultrathin MnO2 decorated graphene/carbon nanotube nanocomposites as efficient sulfur hosts for high-performance lithium-sulfur batteries.

Project description:Lithium-sulfur batteries are attracting significant attention due to their high theoretical specific capacity and low cost. However, their applications are hindered by the poor conductivity of sulfur and capacity fading caused by the shuttle effect. Here, ultrathin manganese dioxide decorated graphene/carbon nanotube nanocomposites are designed as sulfur hosts to suppress the shuttle effect and improve the adsorption efficiency of polysulfides. The graphene/carbon nanotube hybrids, with extraordinary conductivity and large surface area, function as excellent channels for electron transfer and lithium ion diffusion. The ultrathin manganese dioxide nanosheets enable efficient chemical interaction with polysulfides and promote the redox kinetics of polysulfides. As a result, an ultrathin manganese dioxide decorated graphene/carbon nanotube sulfur composite with high sulfur content (81.8 wt%) delivers a high initial specific capacity of 1015.1 mA h g-1 at a current density of 0.1C, high coulombic efficiency approaching 100% and high capacity retention of 84.1% after 100 cycles. The nanocomposites developed in this work have promising applications in high-performance lithium-sulfur batteries.

| S-EPMC9060960 | biostudies-literature

Human iPS Cells Loaded with MnO2-Based Nanoprobes for Photodynamic and Simultaneous Enhanced Immunotherapy Against Cancer.

Project description: Not available

| S-EPMC7770927 | biostudies-literature

Seminaphthofluorescein-based fluorescent probes for imaging nitric oxide in live cells.

Project description:Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic conditions to yield a 20-45-fold increase in integrated emission. The seminaphthofluorescein-based probes emit at longer wavelengths than the parent FL1 and FL2 fluorescein-based generations of NO probes, maintaining emission maxima between 550 and 625 nm. The emission profiles depend on the excitation wavelength; maximum fluorescence turn-on is achieved at excitations between 535 and 575 nm. The probes are highly selective for NO over other biologically relevant reactive nitrogen and oxygen species including NO(3)(-), NO(2)(-), HNO, ONOO(-), NO(2), OCl(-), and H(2)O(2). The seminaphthofluorescein-based probes can be used to visualize endogenously produced NO in live cells, as demonstrated using Raw 264.7 macrophages.

| S-EPMC3183332 | biostudies-literature

Imaging multiple microRNAs in living cells using ATP self-powered strand-displacement cascade amplification.

Project description:Herein, we design a smart autonomous ATP self-powered strand-displacement cascade amplification (SDCA) system for highly sensitive multiple intracellular miRNA detection. Rationally engineered Y-motif DNA structures are functionalized on mesoporous silica-coated copper sulfide nanoparticles loaded with numerous ATPs (CuS@mSiO2-Y/ATP) through pH stimulus-responsive disulfide bonds. The SDCA system is implemented by endogenous specific miRNA as a trigger and ATP as fuel released from the nanocarrier at acidic pH and photothermal stimuli-responsive CuS. The ATP self-powered SDCA process presents higher sensitivity compared to that without amplification for intracellular miRNA imaging. Two-color simultaneous and sensitive imaging of multiple cancer-related miRNAs in living cells is also confirmed. This enables facile and accurate differentiation between normal cells and different types of cancer cell using intracellular miRNA imaging, which improves the veracity and timeliness for early cancer diagnosis.

| S-EPMC5885591 | biostudies-literature

Fabrication of Na and K based MnO2 nanocomposites for supercapacitive applications.

Project description:α-Na0.5Mn0.93O2@Na0.91MnO2 (NaMnO) and K0.48Mn1.94O5@Na0.91MnO2 (KNaMnO) nanocomposites have been synthesized using solid-state reaction method. FESEM results convey the formation of column-shaped morphology. FTIR exhibited a shift in the vibration frequency upon potassium loading. Cyclic voltammetric curves are scanned (0 V-0.8 V) at different scan rates (5 mV/s to 100 mV/s) in 1M KOH electrolyte. Galvanostatic charge-discharge characteristics, for different current densities, have shown non-linear or pseudocapacitive characteristics of the prepared electrodes. High specific capacitance of ∼361 F/g and ∼143 F/g, at a current density of 1A/g, has been achieved for KNaMnO and NaMnO samples, respectively. KNaMnO sample exhibited higher capacitive retention (116 %), up to 2000 cycles, and obeys lower series resistance, charge transfer resistance, and Warburg impedance parameters, thus, convey higher efficiency of this compound for supercapacitor applications.

| S-EPMC11334620 | biostudies-literature