Unknown

Dataset Information

0

Seminaphthofluorescein-based fluorescent probes for imaging nitric oxide in live cells.


ABSTRACT: Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic conditions to yield a 20-45-fold increase in integrated emission. The seminaphthofluorescein-based probes emit at longer wavelengths than the parent FL1 and FL2 fluorescein-based generations of NO probes, maintaining emission maxima between 550 and 625 nm. The emission profiles depend on the excitation wavelength; maximum fluorescence turn-on is achieved at excitations between 535 and 575 nm. The probes are highly selective for NO over other biologically relevant reactive nitrogen and oxygen species including NO(3)(-), NO(2)(-), HNO, ONOO(-), NO(2), OCl(-), and H(2)O(2). The seminaphthofluorescein-based probes can be used to visualize endogenously produced NO in live cells, as demonstrated using Raw 264.7 macrophages.

SUBMITTER: Pluth MD 

PROVIDER: S-EPMC3183332 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Seminaphthofluorescein-based fluorescent probes for imaging nitric oxide in live cells.

Pluth Michael D MD   Chan Maria R MR   McQuade Lindsey E LE   Lippard Stephen J SJ  

Inorganic chemistry 20110907 19


Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic conditions to yield a 20-45-fold increase in integrated emission. The seminaphthofluorescein-based probes emit at longer wavelengths than the parent FL1 and FL2 fluorescein-based generations of NO probes, maintaining emission maxima between 550 and 625 nm. The emission profiles depen  ...[more]

Similar Datasets

| S-EPMC4743004 | biostudies-literature
| S-EPMC2871341 | biostudies-literature
| S-EPMC9904808 | biostudies-literature
| S-EPMC4970878 | biostudies-literature
| S-EPMC9795370 | biostudies-literature
| S-EPMC6804920 | biostudies-literature
| S-EPMC3927988 | biostudies-literature
| S-EPMC6102254 | biostudies-other
| S-EPMC4915154 | biostudies-literature
| S-EPMC6453474 | biostudies-literature