Dataset Information

Molecular basis for DPY-30 association to COMPASS-like and NURF complexes.

ABSTRACT: DPY-30 is a subunit of mammalian COMPASS-like complexes (complex of proteins associated with Set1) and regulates global histone H3 Lys-4 trimethylation. Here we report structural evidence showing that the incorporation of DPY-30 into COMPASS-like complexes is mediated by several hydrophobic interactions between an amphipathic ? helix located on the C terminus of COMPASS subunit ASH2L and the inner surface of the DPY-30 dimerization/docking (D/D) module. Mutations impairing the interaction between ASH2L and DPY-30 result in a loss of histone H3K4me3 at the ? locus control region and cause a delay in erythroid cell terminal differentiation. Using overlay assays, we defined a consensus sequence for DPY-30 binding proteins and found that DPY-30 interacts with BAP18, a subunit of the nucleosome remodeling factor complex. Overall, our results indicate that the ASH2L/DPY-30 complex is important for cell differentiation and provide insights into the ability of DPY-30 to associate with functionally divergent multisubunit complexes.

SUBMITTER: Tremblay V

PROVIDER: S-EPMC5832440 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Molecular basis for DPY-30 association to COMPASS-like and NURF complexes.

Tremblay Véronique V Zhang Pamela P Chaturvedi Chandra-Prakash CP Thornton Janet J Brunzelle Joseph S JS Skiniotis Georgios G Shilatifard Ali A Brand Marjorie M Couture Jean-François JF

Structure (London, England : 1993) 20141120 12

DPY-30 is a subunit of mammalian COMPASS-like complexes (complex of proteins associated with Set1) and regulates global histone H3 Lys-4 trimethylation. Here we report structural evidence showing that the incorporation of DPY-30 into COMPASS-like complexes is mediated by several hydrophobic interactions between an amphipathic α helix located on the C terminus of COMPASS subunit ASH2L and the inner surface of the DPY-30 dimerization/docking (D/D) module. Mutations impairing the interaction betwee ...[more]

PMID: 25456412

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Dataset Information

Molecular basis for DPY-30 association to COMPASS-like and NURF complexes.

Publications

Molecular basis for DPY-30 association to COMPASS-like and NURF complexes.

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