Ontology highlight
ABSTRACT:
SUBMITTER: Park SY
PROVIDER: S-EPMC5832519 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Park Sung Young SY Song Sang-Ho SH Palmateer Brandon B Pal Akash A Petersen Eric D ED Shall Gabrielle P GP Welchko Ryan M RM Ibata Keiji K Miyawaki Atsushi A Augustine George J GJ Hochgeschwender Ute U
Journal of neuroscience research 20170901 3
Previous work has demonstrated that fusion of a luciferase to an opsin, to create a luminescent opsin or luminopsin, provides a genetically encoded means of manipulating neuronal activity via both chemogenetic and optogenetic approaches. Here we have expanded and refined the versatility of luminopsin tools by fusing an alternative luciferase variant with high light emission, Gaussia luciferase mutant GLucM23, to depolarizing and hyperpolarizing channelrhodopsins with increased light sensitivity. ...[more]