Unknown

Dataset Information

0

Discovery of Shoot Branching Regulator Targeting Strigolactone Receptor DWARF14.


ABSTRACT: DWARF14 (D14) is a strigolactone receptor that plays a central role in suppression of shoot branching, and hence is a potential target to increase crop productions and biomass. Recently, we reported a fluorescence turn-on probe, Yoshimulactone Green (YLG), which generates a strong fluorescence upon the hydrolysis by D14-type strigolactone receptors. Herein, we applied a YLG-based in vitro assay to a high-throughput chemical screening and identified a novel small molecule DL1 as a potent inhibitor of D14. DL1 competes with endogenous strigolactones, thereby increasing the number of shoot branching in a model plant Arabidopsis as well as in rice. Thus, DL1 is expected to be useful not only as a tool to understand the biological roles of D14 receptors in plant growth and development, but also as a potent agrochemical to improve the crop yield.

SUBMITTER: Yoshimura M 

PROVIDER: S-EPMC5833010 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Discovery of Shoot Branching Regulator Targeting Strigolactone Receptor DWARF14.

Yoshimura Masahiko M   Sato Ayato A   Kuwata Keiko K   Inukai Yoshiaki Y   Kinoshita Toshinori T   Itami Kenichiro K   Tsuchiya Yuichiro Y   Hagihara Shinya S  

ACS central science 20180207 2


DWARF14 (D14) is a strigolactone receptor that plays a central role in suppression of shoot branching, and hence is a potential target to increase crop productions and biomass. Recently, we reported a fluorescence turn-on probe, Yoshimulactone Green (YLG), which generates a strong fluorescence upon the hydrolysis by D14-type strigolactone receptors. Herein, we applied a YLG-based <i>in vitro</i> assay to a high-throughput chemical screening and identified a novel small molecule DL1 as a potent i  ...[more]

Similar Datasets

| S-EPMC2892150 | biostudies-literature
| S-EPMC5913616 | biostudies-literature
| S-EPMC6331613 | biostudies-literature
| S-EPMC5913607 | biostudies-literature
| S-EPMC6896703 | biostudies-literature
| S-EPMC8948499 | biostudies-literature
| S-EPMC4566059 | biostudies-literature
| S-EPMC4650425 | biostudies-literature
| S-EPMC6433298 | biostudies-literature
| S-EPMC7748003 | biostudies-literature