Dataset Information

Strigolactone perception and deactivation by a hydrolase receptor DWARF14.

ABSTRACT: The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an ?/?-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14D218A catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.

SUBMITTER: Seto Y

PROVIDER: S-EPMC6331613 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Strigolactone perception and deactivation by a hydrolase receptor DWARF14.

Seto Yoshiya Y Yasui Rei R Kameoka Hiromu H Tamiru Muluneh M Cao Mengmeng M Terauchi Ryohei R Sakurada Akane A Hirano Rena R Kisugi Takaya T Hanada Atsushi A Umehara Mikihisa M Seo Eunjoo E Akiyama Kohki K Burke Jason J Takeda-Kamiya Noriko N Li Weiqiang W Hirano Yoshinori Y Hakoshima Toshio T Mashiguchi Kiyoshi K Noel Joseph P JP Kyozuka Junko J Yamaguchi Shinjiro S

Nature communications 20190114 1

The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14<sup>D218A</sup> catalytic mutant that lacks enzymatic activity is still able to com ...[more]

PMID: 30643123

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Project description:BackgroundStrigolactones (SLs) are recently identified plant hormones modulating root and shoot branching. Besides their endogenous role within the producing organism, SLs are also key molecules in the communication of plants with arbuscular mycorrhizal (AM) fungi and parasitic weeds. In fact SLs are exuded into the rhizosphere where they act as a host-derived signal, stimulating the germination of the seeds of parasitic plants which would not survive in the absence of a host root to colonize. Similarly, their perception by AM fungi causes extensive hyphal branching; this is a prerequisite for effective root colonization, since it increases the number of potential contact points with the host surface. In spite of the crucial and multifaceted biological role of SLs, there is no information on the receptor(s) which bind(s) such active molecules, neither in the producing plants, or in parasitic weeds or AM fungi.ResultsIn this work, we applied homology modelling techniques to investigate the structure of the protein encoded by the gene Dwarf14, which was first identified in rice as conferring SLs insensitivity when mutated. The best sequence identity was with bacterial RsbQ. Both proteins belong to the superfamily of alpha/beta-fold hydrolases, some members of which play a role in the metabolism or signalling of plant hormones. The Dwarf14 (D14) structure was refined by means of molecular dynamics simulations. In order to support the hypothesis that D14 could be an endogenous SLs receptor, we performed docking experiments with a natural ligand.ConclusionsIt is suggested that D14 interacts with and thereby may act as a receptor for SLs in plants. This hypothesis offers a starting point to experimentally study the mechanism of its activity in vivo by means of structural, molecular and genetic approaches. Lastly, knowledge of the putative receptor structure will boost the research on analogues of the natural substrates as required for agricultural applications.

Dataset Information

Strigolactone perception and deactivation by a hydrolase receptor DWARF14.

Publications

Strigolactone perception and deactivation by a hydrolase receptor DWARF14.

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