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Overexpression of Cu-Zn SOD in Brucella abortus suppresses bacterial intracellular replication via down-regulation of Sar1 activity.

ABSTRACT: Brucella Cu-Zn superoxide dismutase (Cu-Zn SOD) is a periplasmic protein, and immunization of mice with recombinant Cu-Zn SOD protein confers protection against Brucella abortus infection. However, the role of Cu-Zn SOD during the process of Brucella infection remains unknown. Here, we report that Cu-Zn SOD is secreted into culture medium and is translocated into host cells independent of type IV secretion systems (T4SS). Furthermore, co-immunoprecipitation and immunofluorescence studies reveal that Brucella abortus Cu-Zn SOD interacts with the small GTPase Sar1. Overexpression of Cu-Zn SOD in Brucella abortus inhibits bacterial intracellular growth by abolishing Sar1 activity in a manner independent of reactive oxygen species (ROS) production.

SUBMITTER: Liu X 

PROVIDER: S-EPMC5839387 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Overexpression of Cu-Zn SOD in <i>Brucella abortus</i> suppresses bacterial intracellular replication via down-regulation of Sar1 activity.

Liu Xiaofeng X   Zhou Mi M   Yang Yanling Y   Wu Jing J   Peng Qisheng Q  

Oncotarget 20180110 11

<i>Brucella</i> Cu-Zn superoxide dismutase (Cu-Zn SOD) is a periplasmic protein, and immunization of mice with recombinant Cu-Zn SOD protein confers protection against <i>Brucella abortus</i> infection. However, the role of Cu-Zn SOD during the process of <i>Brucella</i> infection remains unknown. Here, we report that Cu-Zn SOD is secreted into culture medium and is translocated into host cells independent of type IV secretion systems (T4SS). Furthermore, co-immunoprecipitation and immunofluores  ...[more]

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