Ontology highlight
ABSTRACT:
SUBMITTER: Yayon N
PROVIDER: S-EPMC5844907 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Yayon Nadav N Dudai Amir A Vrieler Nora N Amsalem Oren O London Michael M Soreq Hermona H
Scientific reports 20180309 1
Three-dimensional structures in biological systems are routinely evaluated using large image stacks acquired from fluorescence microscopy; however, analysis of such data is muddled by variability in the signal across and between samples. Here, we present Intensify3D: a user-guided normalization algorithm tailored for overcoming common heterogeneities in large image stacks. We demonstrate the use of Intensify3D for analyzing cholinergic interneurons of adult murine brains in 2-Photon and Light-Sh ...[more]