Ontology highlight
ABSTRACT:
SUBMITTER: Benoit-Pilven C
PROVIDER: S-EPMC5844962 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Benoit-Pilven Clara C Marchet Camille C Chautard Emilie E Lima Leandro L Lambert Marie-Pierre MP Sacomoto Gustavo G Rey Amandine A Cologne Audric A Terrone Sophie S Dulaurier Louis L Claude Jean-Baptiste JB Bourgeois Cyril F CF Auboeuf Didier D Lacroix Vincent V
Scientific reports 20180309 1
Genome-wide analyses estimate that more than 90% of multi exonic human genes produce at least two transcripts through alternative splicing (AS). Various bioinformatics methods are available to analyze AS from RNAseq data. Most methods start by mapping the reads to an annotated reference genome, but some start by a de novo assembly of the reads. In this paper, we present a systematic comparison of a mapping-first approach (FARLINE) and an assembly-first approach (KISSPLICE). We applied these meth ...[more]