Unknown

Dataset Information

0

RAGE-specific single chain Fv for PET imaging of pancreatic cancer.


ABSTRACT: Noninvasive detection of both early pancreatic neoplasia and metastases could enhance strategies to improve patient survival in this disease that is notorious for an extremely poor prognosis. There are almost no identifiable targets for non-invasive diagnosis by positron emission tomography (PET) for patients with pancreatic ductal adenocarcinoma (PDAC). Over-expression of the receptor for advanced glycation end products (RAGE) is found on the cell surface of both pre-neoplastic lesions and invasive PDAC. Here, a RAGE-specific single chain (scFv) was developed, specific for PET imaging in syngeneic mouse models of PDAC. An anti-RAGE scFv conjugated with a sulfo-Cy5 fluorescence molecule showed high affinity and selectivity for RAGE expressing pancreatic tumor cells and genetically engineered KRASG12D mouse models of PDAC. An in vivo biodistribution study was performed with the 64Cu-radiolabled scFv in a syngeneic murine pancreatic cancer model, demonstrating both the feasibility and potential of an anti-RAGE scFv for detection of PDAC. These studies hold great promise for translation into the clinic.

SUBMITTER: Kim HY 

PROVIDER: S-EPMC5846720 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

RAGE-specific single chain Fv for PET imaging of pancreatic cancer.

Kim Hye-Yeong HY   Wang Xiaolei X   Kang Rui R   Tang Daolin D   Boone Brian A BA   Zeh Herbert J HJ   Lotze Michael T MT   Edwards W Barry WB  

PloS one 20180312 3


Noninvasive detection of both early pancreatic neoplasia and metastases could enhance strategies to improve patient survival in this disease that is notorious for an extremely poor prognosis. There are almost no identifiable targets for non-invasive diagnosis by positron emission tomography (PET) for patients with pancreatic ductal adenocarcinoma (PDAC). Over-expression of the receptor for advanced glycation end products (RAGE) is found on the cell surface of both pre-neoplastic lesions and inva  ...[more]

Similar Datasets

| S-EPMC7172176 | biostudies-literature
| S-EPMC7901706 | biostudies-literature
| S-EPMC15200 | biostudies-literature
| S-EPMC1183604 | biostudies-literature
| S-EPMC3255864 | biostudies-other
| S-EPMC7591811 | biostudies-literature
| S-EPMC6151396 | biostudies-literature
| S-EPMC92415 | biostudies-literature
| S-EPMC6681014 | biostudies-literature
| S-EPMC2630973 | biostudies-literature