CRISPR RNAs trigger innate immune responses in human cells.
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ABSTRACT:
SUBMITTER: Kim S
PROVIDER: S-EPMC5848615 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Publications
CRISPR RNAs trigger innate immune responses in human cells.
Kim Sojung S Koo Taeyoung T Jee Hyeon-Gun HG Cho Hee-Yeon HY Lee Gyeorae G Lim Dong-Gyun DG Shin Hyoung Shik HS Kim Jin-Soo JS
Genome research 20180301 3
Here, we report that CRISPR guide RNAs (gRNAs) with a 5'-triphosphate group (5'-ppp gRNAs) produced via in vitro transcription trigger RNA-sensing innate immune responses in human and murine cells, leading to cytotoxicity. 5'-ppp gRNAs in the cytosol are recognized by DDX58, which in turn activates type I interferon responses, causing up to ∼80% cell death. We show that the triphosphate group can be removed by a phosphatase in vitro and that the resulting 5'-hydroxyl gRNAs in complex with Cas9 o ...[more]
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