Ontology highlight
ABSTRACT:
SUBMITTER: Schieferstein JM
PROVIDER: S-EPMC5849577 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Schieferstein Jeremy M JM Pawate Ashtamurthy S AS Varel Michael J MJ Guha Sudipto S Astrauskaite Ieva I Gennis Robert B RB Kenis Paul J A PJA
Lab on a chip 20180301 6
Crystallization of membrane proteins is a critical step for uncovering atomic resolution 3-D structures and elucidating structure-function relationships. Microseeding, the process of transferring sub-microscopic crystal nuclei from initial screens into new crystallization experiments, is an effective, yet underutilized approach to grow crystals suitable for X-ray crystallography. Here, we report simplified methods for crystallization of membrane proteins that utilize microseeding in X-ray transp ...[more]