Unknown

Dataset Information

0

Mobilization of progenitor cells and assessment of vessel healing after second generation drug-eluting stenting by optical coherence tomography.


ABSTRACT:

Background

Bone marrow-derived progenitor cells likely contribute to both endothelial- and smooth muscle cell-dependent healing responses in stent-injured vessel sites. This study aimed to assess mobilization of progenitor cells and vessel healing after zotarolimus-eluting (ZES) and everolimus-eluting (EES) stents.

Methods and results

In 63 patients undergoing coronary stent implantation, we measured circulating CD34 + CD133 + CD45low cells and serum levels of biomarkers relevant to stem cell mobilization. In 31 patients of them, we assessed vessel healing within the stented segment using optical coherence tomography (OCT) imaging. The CD34 + CD133 + CD45low cells increased 68 ± 59% 7 days after bare metal stent (BMS), 10 ± 53% after ZES (P < 0.01 vs BMS), 3 ± 49% after EES (P < 0.001 vs BMS), compared with baseline. Percent change in CD34 + CD133 + CD45low cells was positively correlated with that in stromal cell-derived factor (SDF)-1? (R = 0.29, P = 0.034). Percentage of uncovered struts was higher in the EES group (14.4 ± 17.3%), compared with the BMS (0.7 ± 1.3, P < 0.01) and ZES (0.4 ± 0.5, P < 0.01) groups. The change in CD34 + CD133 + CD45low cells showed positive correlation with OCT-quantified mean neointimal area (R = 0.48, P < 0.01). Finally, circulating mononuclear cells obtained from 5 healthy volunteers were isolated to determine the effect of sirolimus, zotarolimus and everolimus on vascular cell differentiation. The differentiation of mononuclear cells into endothelial-like cells was dose-dependently suppressed by sirolimus, zotarolimus, and everolimus.

Conclusions

Mobilization of progenitor cells was suppressed, and differentiation of mononuclear cells into endothelial-like cells was inhibited, in association with increased number of uncovered stent struts, even after second generation drug-eluting stenting. These data suggest that new approaches are necessary to enhance stent healing.

SUBMITTER: Sakuma M 

PROVIDER: S-EPMC5854838 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mobilization of progenitor cells and assessment of vessel healing after second generation drug-eluting stenting by optical coherence tomography.

Sakuma Masashi M   Nasuno Takahisa T   Abe Shichiro S   Obi Syotaro S   Toyoda Shigeru S   Taguchi Isao I   Sohma Ryoichi R   Inoue Ken-Ichi KI   Nishino Setsu S   Node Koichi K   Attizzani Guiherme G   Bezerra Hiram H   Costa Marco M   Simon Daniel D   Inoue Teruo T  

International journal of cardiology. Heart & vasculature 20180216


<h4>Background</h4>Bone marrow-derived progenitor cells likely contribute to both endothelial- and smooth muscle cell-dependent healing responses in stent-injured vessel sites. This study aimed to assess mobilization of progenitor cells and vessel healing after zotarolimus-eluting (ZES) and everolimus-eluting (EES) stents.<h4>Methods and results</h4>In 63 patients undergoing coronary stent implantation, we measured circulating CD34 + CD133 + CD45low cells and serum levels of biomarkers relevant  ...[more]

Similar Datasets

| S-EPMC11336043 | biostudies-literature
| S-EPMC4709546 | biostudies-literature
| S-EPMC8965003 | biostudies-literature
| S-EPMC6333625 | biostudies-literature
| S-EPMC9692107 | biostudies-literature
| S-EPMC8215158 | biostudies-literature
| S-EPMC5650189 | biostudies-literature
| S-EPMC8361292 | biostudies-literature
| S-EPMC5430649 | biostudies-literature
| S-EPMC6791857 | biostudies-literature