Ontology highlight
ABSTRACT:
SUBMITTER: Adelmund SM
PROVIDER: S-EPMC5856642 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
ACS chemical biology 20180208 3
This work introduces light-activated bioorthogonal noncanonical amino acid tagging (laBONCAT) as a method to selectively label, isolate, and identify proteins newly synthesized at user-defined regions in tissue culture. By photocaging l-azidohomoalanine (Aha), metabolic incorporation into proteins is prevented. The caged compound remains stable for many hours in culture, but can be photochemically liberated rapidly and on demand with spatial control. Upon directed light exposure, the uncaged ami ...[more]