Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis.

ABSTRACT: The aim of this study is to elucidate the biogenetic modification of donor and recipient interleukin-28B (IL-28B) genotypes in liver graft biopsies after living donor liver transplantation (LDLT) for chronic hepatitis C virus- (HCV-) related, end-stage liver disease. Fifty liver graft biopsies were collected from recipients during LDLT treatment for HCV-related, end-stage liver disease. DNA was extracted from all 50 liver tissues, and the IL-28B single-nucleotide polymorphisms (SNPs) rs8099917 and rs12979860 were studied for allelic discrimination by real-time PCR analysis. Blood samples were obtained from donors and recipients on postoperative day 0 (POD0), POD7, and POD30. We randomly selected five liver biopsies and isolated the hepatocytes by laser capture microdissection (LCM) to evaluate genotype modifications resulting from LDLT. After LDLT, the IL-28B SNP rs8099917 was identified not only in the liver graft biopsies and donors' sera (TT = 41?:?43; GT = 9?:?5; GG = 0?:?2), but also in liver graft biopsies and recipients' sera on POD0 (TT = 41?:?44; GT = 9?:?4; GG = 0?:?2), POD7 (TT = 41?:?30; GT = 9?:?18; GG = 0?:?2), and POD30 (TT = 41?:?29; GT = 9?:?19; GG = 0?:?2). A significant difference was observed between the rs8099917 allele frequencies of liver graft biopsies and recipients' sera on POD30 (p = 0.039). In addition, a significant difference was also noted between the rs12979860 allele frequencies of liver graft biopsies and donors' sera (CT = 49?:?39; TT = 1?:?10) (p = 0.012) and of liver graft biopsies and recipients' sera on POD0 (CT = 49?:?39; TT = 1?:?11) (p = 0.002), POD7 (CT = 49?:?42; TT = 1?:?8) (p = 0.016), and POD30 (CT = 49?:?41; TT = 1?:?9) (p = 0.008). This phenomenon was confirmed by pyrosequencing of hepatocytes isolated by LCM. Following LDLT, the TT-to-GT IL-28B genotype modification predominated in rs8099917, and the CC-to-CT modification predominated in rs12979860. In conclusion, these modified phenomena suggested that the selected donor with a predictable and favourable IL-28B genotype will not confer a benefit on the recipient in the living donor liver transplantation setting.

SUBMITTER: Chiu KW 

PROVIDER: S-EPMC5857340 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature