Capillary pericytes express ?-smooth muscle actin, which requires prevention of filamentous-actin depolymerization for detection.

ABSTRACT: Recent evidence suggests that capillary pericytes are contractile and play a crucial role in the regulation of microcirculation. However, failure to detect components of the contractile apparatus in capillary pericytes, most notably ?-smooth muscle actin (?-SMA), has questioned these findings. Using strategies that allow rapid filamentous-actin (F-actin) fixation (i.e. snap freeze fixation with methanol at -20°C) or prevent F-actin depolymerization (i.e. with F-actin stabilizing agents), we demonstrate that pericytes on mouse retinal capillaries, including those in intermediate and deeper plexus, express ?-SMA. Junctional pericytes were more frequently ?-SMA-positive relative to pericytes on linear capillary segments. Intravitreal administration of short interfering RNA (?-SMA-siRNA) suppressed ?-SMA expression preferentially in high order branch capillary pericytes, confirming the existence of a smaller pool of ?-SMA in distal capillary pericytes that is quickly lost by depolymerization. We conclude that capillary pericytes do express ?-SMA, which rapidly depolymerizes during tissue fixation thus evading detection by immunolabeling.

SUBMITTER: Alarcon-Martinez L 

PROVIDER: S-EPMC5862523 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature