Ontology highlight
ABSTRACT:
SUBMITTER: Chen D
PROVIDER: S-EPMC5862989 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Chen DaYang D Zhen HeFu H Qiu Yong Y Liu Ping P Zeng Peng P Xia Jun J Shi QianYu Q Xie Lin L Zhu Zhu Z Gao Ya Y Huang GuoDong G Wang Jian J Yang HuanMing H Chen Fang F
Scientific reports 20180321 1
Research based on a strategy of single-cell low-coverage whole genome sequencing (SLWGS) has enabled better reproducibility and accuracy for detection of copy number variations (CNVs). The whole genome amplification (WGA) method and sequencing platform are critical factors for successful SLWGS (<0.1 × coverage). In this study, we compared single cell and multiple cells sequencing data produced by the HiSeq2000 and Ion Proton platforms using two WGA kits and then comprehensively evaluated the GC- ...[more]