Rapid and dynamic arginylation of the leading edge ?-actin is required for cell migration.

ABSTRACT: ?-actin plays key roles in cell migration. Our previous work demonstrated that ?-actin in migratory non-muscle cells is N-terminally arginylated and that this arginylation is required for normal lamellipodia extension. Here, we examined the function of ?-actin arginylation in cell migration. We found that arginylated ?-actin is concentrated at the leading edge of lamellipodia and that this enrichment is abolished after serum starvation as well as in contact-inhibited cells in confluent cultures, suggesting that arginylated ?-actin at the cell leading edge is coupled to active migration. Arginylated actin levels exhibit dynamic changes in response to cell stimuli, lowered after serum starvation and dramatically elevating within minutes after cell stimulation by readdition of serum or lysophosphatidic acid. These dynamic changes require active translation and are not seen in confluent contact-inhibited cell cultures. Microinjection of arginylated actin antibodies into cells severely and specifically inhibits their migration rates. Together, these data strongly suggest that arginylation of ?-actin is a tightly regulated dynamic process that occurs at the leading edge of locomoting cells in response to stimuli and is integral to the signaling network that regulates cell migration.

SUBMITTER: Pavlyk I 

PROVIDER: S-EPMC5869130 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature