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PMAnalyzer: a new web interface for bacterial growth curve analysis.


ABSTRACT:

Summary

Bacterial growth curves are essential representations for characterizing bacteria metabolism within a variety of media compositions. Using high-throughput, spectrophotometers capable of processing tens of 96-well plates, quantitative phenotypic information can be easily integrated into the current data structures that describe a bacterial organism. The PMAnalyzer pipeline performs a growth curve analysis to parameterize the unique features occurring within microtiter wells containing specific growth media sources. We have expanded the pipeline capabilities and provide a user-friendly, online implementation of this automated pipeline. PMAnalyzer version 2.0 provides fast automatic growth curve parameter analysis, growth identification and high resolution figures of sample-replicate growth curves and several statistical analyses.

Availability and implementation

PMAnalyzer v2.0 can be found at https://edwards.sdsu.edu/pmanalyzer/ . Source code for the pipeline can be found on GitHub at https://github.com/dacuevas/PMAnalyzer . Source code for the online implementation can be found on GitHub at https://github.com/dacuevas/PMAnalyzerWeb .

Contact

dcuevas08@gmail.com.

Supplementary information

Supplementary data are available at Bioinformatics online.

SUBMITTER: Cuevas DA 

PROVIDER: S-EPMC5870709 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Publications

PMAnalyzer: a new web interface for bacterial growth curve analysis.

Cuevas Daniel A DA   Edwards Robert A RA  

Bioinformatics (Oxford, England) 20170601 12


<h4>Summary</h4>Bacterial growth curves are essential representations for characterizing bacteria metabolism within a variety of media compositions. Using high-throughput, spectrophotometers capable of processing tens of 96-well plates, quantitative phenotypic information can be easily integrated into the current data structures that describe a bacterial organism. The PMAnalyzer pipeline performs a growth curve analysis to parameterize the unique features occurring within microtiter wells contai  ...[more]

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