Ontology highlight
ABSTRACT:
SUBMITTER: Reynolds EW
PROVIDER: S-EPMC5875912 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Reynolds Evan W EW Schwochert Timothy D TD McHenry Matthew W MW Watters John W JW Brustad Eric M EM
Chembiochem : a European journal of chemical biology 20171109 24
A cytochrome P450 was engineered to selectively incorporate Ir(Me)-deuteroporphyrin IX (Ir(Me)-DPIX), in lieu of heme, in bacterial cells. Cofactor selectivity was altered by introducing mutations within the heme-binding pocket to discriminate the deuteroporphyrin macrocycle, in combination with mutations to the P450 axial cysteine to accommodate a pendant methyl group on the Ir(Me) center. This artificial metalloenzyme was investigated for activity in non-native metallocarbenoid-mediated olefin ...[more]