A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria.
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ABSTRACT:
SUBMITTER: Chang HJ
PROVIDER: S-EPMC5880506 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria.
Chang Hung-Ju HJ Mayonove Pauline P Zavala Agustin A De Visch Angelique A Minard Philippe P Cohen-Gonsaud Martin M Bonnet Jerome J
ACS synthetic biology 20171030 1
Engineered bacteria promise to revolutionize diagnostics and therapeutics, yet many applications are precluded by the limited number of detectable signals. Here we present a general framework to engineer synthetic receptors enabling bacterial cells to respond to novel ligands. These receptors are activated via ligand-induced dimerization of a single-domain antibody fused to monomeric DNA-binding domains (split-DBDs). Using E. coli as a model system, we engineer both transmembrane and cytosolic r ...[more]
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