Cross-reactivity of anti-HMGB1 antibodies for HMGB2.
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ABSTRACT:
SUBMITTER: Davies JE
PROVIDER: S-EPMC5886380 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
Cross-reactivity of anti-HMGB1 antibodies for HMGB2.
Davies Jessica E JE Apta Bonita H R BHR Harper Matthew T MT
Journal of immunological methods 20180215
HMGB1 and HMGB2 are DNA-interacting proteins but can also have extracellular actions during inflammation. Despite their relatively high homology, they may have distinct roles, making it essential to be able to differentiate between the two. Here we examine the specificity of five commercially-available anti-HMGB1 antibodies. By Western blotting of recombinant proteins and HMGB1-/- mouse embryonic fibroblasts, we identified only one HMGB1 antibody that, under our experimental conditions, did not ...[more]
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