Ontology highlight
ABSTRACT:
SUBMITTER: Lee H
PROVIDER: S-EPMC5889325 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Lee Hayun H Zhou Yi Y Taylor David W DW Sashital Dipali G DG
Molecular cell 20180327 1
CRISPR-Cas immune systems integrate short segments of foreign DNA as spacers into the host CRISPR locus to provide molecular memory of infection. Cas4 proteins are widespread in CRISPR-Cas systems and are thought to participate in spacer acquisition, although their exact function remains unknown. Here we show that Bacillus halodurans type I-C Cas4 is required for efficient prespacer processing prior to Cas1-Cas2-mediated integration. Cas4 interacts tightly with the Cas1 integrase, forming a hete ...[more]