Ontology highlight
ABSTRACT:
SUBMITTER: Chavez A
PROVIDER: S-EPMC5889643 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Chavez Alejandro A Pruitt Benjamin W BW Tuttle Marcelle M Shapiro Rebecca S RS Cecchi Ryan J RJ Winston Jordan J Turczyk Brian M BM Tung Michael M Collins James J JJ Church George M GM Church George M GM
Proceedings of the National Academy of Sciences of the United States of America 20180319 14
Here, we present a generalized method of guide RNA "tuning" that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undesired gain-of-function mutations within a population of engineered organisms. We further demonstrate that the system is scalable to a multitude of targets and that the general tuning and prevention con ...[more]