Ontology highlight
ABSTRACT:
SUBMITTER: Bashir S
PROVIDER: S-EPMC7585302 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Bashir Sanum S Dang Tu T Rossius Jana J Wolf Johanna J Kühn Ralf R
BMC biotechnology 20201023 1
<h4>Background</h4>Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR). Since HDR competes with the prevailing non-homologous end joining (NHEJ) pathway and depends on the presence of repair templates its efficiency is often limited and demands optimized methodology.<h4>Results</h4>For the enhancement of HDR we redirect the DSB repair pathwa ...[more]